Venus熒光蛋白的激發(fā)光波長是515nm，發(fā)射光波長是529nm左右。Venus熒光蛋白屬于黃色熒光蛋白的一種，所以，Venus熒光蛋白的發(fā)射光光譜圖可參考黃色熒光蛋白的光譜圖。

Venus Fluorescence Protein

Venus is a basic (constitutively fluorescent) yellow fluorescent protein published in 2002, derived from Aequorea victoria. It is reported to be a rapidly-maturing weak dimer with moderate acid sensitivity.

Yellow  Fluorescence Proteins

如果要觀察Venus黃色熒光蛋白（Venus Fluorescent Protein)的表達，美國路陽生產(chǎn)的便攜式熒光蛋白激發(fā)光源可以選擇LUYOR-3260CY和LUYOR-3415C（X）系列雙波長熒光蛋白激發(fā)光源。黃色熒光蛋白采用青色光源激發(fā)，佩戴LUV-40A桔色觀察眼鏡觀察，能夠分選愈傷組織、菌落、植株、種子等黃色熒光蛋白表達陽性的目標，是轉(zhuǎn)基因篩選的良好工具。如希望提供更多詳細信息，可直接聯(lián)系上海路陽生物技術(shù)有限公司的銷售客服。

下圖為用于觀察黃色熒光蛋白的便攜式激發(fā)光源LUYOR-3415:

下圖為用于觀察黃色熒光蛋白的觀察眼鏡LUV-40A：

下圖為用于拍攝黃色熒光蛋白的拍照濾鏡LUV-550A：

Yellow Fluorescent Proteins

Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.