LUYOR-3109高強度紫外催化光源促銷
LUYOR-3109紫外光源采用了9顆365nm大功率led,安裝有二次光學透鏡,輸出紫外線強度高,...
2024-08-08作者:激發光源事業部時間:2019-11-17 08:46:42瀏覽9665 次
FITC激發發射波長激發波長為488nm,發射波長為525nm,FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.
FITC激發發射波長激發波長為488nm,發射波長為525nm
FITC has excitation and emission spectrum peak wavelengths of approximately 495 nm/519 nm, giving it a green color. Like most fluorochromes, it is prone to photobleaching.
Fluorescein (FITC)
Fluorescein derivatives are the most common fluorescent reagents for biological research because of their high absorptivity, excellent fluorescence quantum yield, and good water solubility.
Fluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications. Fluorescein displays:
A relatively high rate of photobleaching
Fluorescent signal that is sensitive to pH changes
A relatively broad fluorescence emission spectrum
Fluorescence quenching on conjugation to biopolymers
Excitation max 490nm,Emission max 525nm
FITC一般指異硫氰酸熒光素,異硫氰酸熒光橙紅,異硫氰酸熒光黃,異硫氰酸熒光紅,異硫氰酸熒光素異構體I,5-異硫氰酸熒光素。其形態為黃色粉末,有吸濕性,是一種生化試劑,也是醫學診斷藥物,可對由地細菌病毒和寄生蟲等所致疾病進行快速診斷。并且它能與各種抗體蛋白結合,結合后的抗體不喪失與一定抗原結合的特異性,并在堿性溶液中仍有強烈綠色熒光,加酸后析出沉淀,熒光消失,微溶于丙酮、乙醚和石油醚。該品是生化試劑,也是醫學診斷藥物,主要用于熒光抗體技術中的熒光染料,能和各種抗體蛋白結合,結合后的抗體不喪失與一定抗原結合的特異性,并在堿性溶液中具有強烈的綠色熒光。用于醫學,農學和畜牧等方面,可對由地細菌病毒和寄生蟲等所致疾病進行快速診斷。
(fluorescein isothiocyanate, FITC) FITC純品為黃色或橙黃色結晶粉末,易溶于水和酒精溶劑。有兩種異構體,其中異構體Ⅰ型在效率、穩定性與蛋白質結合力等方面都更優良。FITC分子量為389.4,更大吸收光波長為490~495nm,更大發射光波長為520~530nm,呈現明亮的黃綠色熒光。FITC在冷暗干燥處可保存多年,是目前應用最廣泛的熒光素。其主要優點是人眼對黃綠色較為敏感,通常切片標本中的綠色熒光少于紅色。危險代碼:Xn,危險等級:R20/21/22-R36/37/38-R42/43。安全等級:S22-S26- S36/37/39-S45。
常見熒光蛋白的激發波長和發射波長:
EBFP2,藍色熒光蛋白,激發波長383nm,發射波長448nm;
mCelulean,青色熒光蛋白,激發波長433nm,發射波長475nm;
EGFP,綠色熒光蛋白,激發波長488nm,發射波長507nm;
EYFP,黃色熒光蛋白,激發波長513nm,發射波長527nm;
mCitrine,黃色熒光蛋白,激發波長516nm,發射波長529nm;
mCherry,紅色熒光蛋白,激發波長587nm,發射波長610nm;
mRuby2,紅色熒光蛋白,激發波長559nm,發射波長600nm;
tdTomato,橙色熒光蛋白,激發波長554nm,發射波長581nm;